肾上腺组织分离手册

　更新时间：2016-02-17　点击量：634

世界*实验材料供应商 Worthington Biochemical 正式授权上海起发为其中国代理， Worthington Biochemical 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海起发一直秉承为中国科研客户带来的产品，的服务，签约 Worthington Biochemical 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Worthington Biochemical 中国代理, Worthington Biochemical 上海代理， Worthington Biochemical 北京代理，Worthington Biochemical 广东代理， Worthington Biochemical 江苏代理Worthington Biochemical 湖北代理,Worthington Biochemical 天津,Worthington Biochemical 黑龙江代理,Worthington Biochemical 内蒙古代理,Worthington Biochemical 吉林代理,Worthington Biochemical 福建代理, Worthington Biochemical 江苏代理, Worthington Biochemical 浙江代理,Worthington Biochemical 四川代理,

Worthington组织消化指南

引言

组织消化酶主要用于组织培养和细胞生物学研究的原代细胞的分离和收集。尽管这些消化酶已经普遍应用多年，但是对其组织消化和细胞收集的作用机制却没有全面的理解。由此导致的结果便是选择一种消化方法大多是随机选择或基于过去的经验，而不是在*理解消化原理的基础上选择的消化方案，以及如何根据具体实验目的修正消化方案以获得更好的实验结果。

细胞分离程序的目的是zui大程度的获得功能活性的单个细胞，而影响结果的原因有很多，包括（不限于）以下：

1、组织类型

2、物种来源

3、动物年龄

4、遗传改造情况（如knockouts）

5、所用的消化培养液

6、所用的酶

7、消化酶中的杂质

8、所用消化酶的工作浓度

9、温度

10、孵育时间

通常前四个原因没办法选择，而剩下的原因一般由经验决定以获取zui适实验结果。

通过查阅文献来选择*组织消化酶和zui适消化条件的研究人员通常被互相矛盾的实验数据搞得一头雾水。这些巨大差异来源于组织胞外基质的复杂而动态的特性，以及过去常用的粗制、未提纯的组织分离酶。另外，组织胞外基质由各种蛋白、糖蛋白、脂类和糖脂组成，这些成分的组成比例在不同物种、不同组织、甚至不同发育（年龄）之间存在明显的差异，这些因素都极大的影响了消化方案的制定。目前通常使用的粗制酶，如链霉蛋白酶、NF1:250、以及含有几种其他不同浓度蛋白酶的胶原酶、以及各种多糖酶、核酶和脂酶。

本指南总结了目前常用组织消化酶的zui适消化和细胞收集知识和方法，描述了标准的实验步骤，以便于制定一个*的细胞分离方案，同时列举了一些相关的参考文献。

下表列出多种组织的适宜消化酶（含参考文献，以组织类型和物种分类，详见链接）

脂肪组织	肾上腺组织	骨组织	脑组织
软骨组织	结肠组织	内皮组织	上皮组织
眼	心脏	肠	肾
肝	肺	淋巴结	乳腺
其它组织	其他组织	肌组织	神经组织
胰腺	腮腺	垂体	前列腺
生殖	动物鳞片	皮肤	脾脏
干细胞	胸腺	甲状腺/甲状旁腺	扁桃体
肿瘤

注：使用Worthington公司消化酶的文献不限于上表链接中所列，一般而言，Worthington公司的组织消化酶可用于大多组织类型的消化。

肾上腺组织分离手册

物种	详细来源	细胞名称	消化用酶	培养基	参考文献
牛	牛	Chromaffin	Collagenase Type 1: 0.1-0.2%	DMEM/F12	Dominguez, N., Rodri璯uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012
			Deoxyribonuclease I: 0.003-0.015%
			Hyaluronidase: 0.015%
牛	牛, 6 个月	Chromaffin	Collagenase Type 1: 0.125%	Locke's solution	Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B: Viability and functionality of b绵羊 chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core, Cell Transplant 15, 121-33, 2006
牛	牛	Chromaffin cells	Collagenase: 0.1%	DMEM	Hahm, S., Chen, Y., Vinson, C. and Eiden, L.: A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization., Mol Pharmacol 64, 1503, 2003
牛	牛	Chromaffin cells	Deoxyribonuclease I: 30 u/ml	DMEM
牛	牛	Chromaffin	Collagenase: 0.2%	Locke's solution	Ortega, J., Sagen, J., and Pappas, G.: Short-term Immunosuppression Enhances Long-term Survival of B绵羊 Chromaffin Cell Xenografts in Rat CNS,Cell Transplant 1, 33, 1992
牛	牛	Chromaffin	Deoxyribonuclease I: 30 u/mg	HEPES	Zhu, J., Li, W., Toews, M., and Hexum, T.: Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in B绵羊 Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process, J Pharmacol Exp Ther 263 (3), 1479, 1992
牛	牛	Heart	Trypsin: 0.06%	25mM HEPES buffered Locke's solution, CMF	Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B绵羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
	(also 大鼠)	Adrenal chromaffin
		Paraneurons
牛	牛	Chromaffin	Collagenase Type 1: 0.25 %	DMEM	Dahmer, M., Hart, P., and Perlman, R.: Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells, J Neurochem 54 (3), 931, 1990
牛	牛	Chromaffin	Collagenase: 0.2%	Locke's solution	Higgins, L., and Berg, D.: Immunological Identification of a Nicotinic Acetylcholine Receptor on B绵羊 Chromaffin Cells, J Neurosci 7 (6), 1792, 1987
牛	牛	Chromaffin	Deoxyribonuclease I: 0.015%	Locke's solution
牛	牛	Chromaffin	Collagenase: 0.05%	Locke's solution, CMF	Aunis, D., Rotllan, P., and Miras-Portugal, M.: Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7, 89, 1985
牛	牛	Chromaffin	Collagenase: 0.15%	Kreb's, CMF	Almazan, G., Aunis, D., Garcia, A., Montiel, C., Nicolas, G., and Sanchez-Garcia, P.: Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells, Br J Biomed Sci 81, 599, 1984
牛	牛	Chromaffin	Collagenase: 0.1%	(see reference)	Pollard, H., Pazoles, C., Creutz, C., Scott, J., Zinder, O., and Hotchkiss, A.:An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells, J Biol Chem 259, 1114, 1984
牛	牛	Chromaffin	Collagenase Type 1: 0.05%	CF Kreb's	Cena, V., Garcia, A., Montiel, C., and Sanchez-Garcia, P.: Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells, Br J Pharmacol 81, 119, 1984
牛	牛	Chromaffin	Collagenase Type 1: 0.025%	HBSS, modified	Waymire, J., Bennett, W., Boehme, R., Hankins, L., Gilmer-Waymire, K., and Haycock, J.: B绵羊 Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture, J Neurosci Methods 7, 329, 1983
牛	牛	Medulla	Hyaluronidase: 0.2%	Saline w/BSA 0.5%	Knight, D. and Baker, P.: Stimulus-Secretion Coupling in Isolated B绵羊 Adrenal Medullary Cells, Q J Exp Physiol 68, 123, 1983
牛	牛	Medulla	Collagenase: 0.2%	Krebs-Ringer bicarbonate buffer, CMF	Greenberg, A. and Zinder, O.: alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells, Cell Tissue Res226, 655, 1982
牛	牛	Chromaffin	Deoxyribonuclease I: 15 礸/ml	Medium A	Wilson, S.P., and Viveros, O.H.: Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium, Exp Cell Res 133, 159, 1981
牛	牛	Chromaffin	Deoxyribonuclease I: 15 礸/ml	(see reference)
牛	牛	Medulla	Protease: 0.2%	Saline	Baker, P., and Knight., D: Calcium Control of Exocytosis and Endocytosis in B绵羊 Adrenal Medullary Cells, Phil Trans R Soc Lond 296, 83, 1981
牛	牛	Chromaffin	Collagenase: 0.05%	Locke's solution, CMF	Trifaro, J.M., and Lee, R.W.: Morphological Characteristics and Stimulus-Secretion Coupling in B绵羊 Adrenal Chromaffin Cell Cultures, Neuroscience 5, 1533, 1980
牛	牛, 成年的	Chromaffin	Collagenase: 0.5%	HBSS	Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980
牛	(还有大鼠, 加拿大-威斯塔, 年幼的; 豚鼠, 初生)	Chromaffin	Collagenase: 0.5%	HBSS
牛	牛	Chromaffin	Collagenase: 0.25%	F-12 medium	Kumakura, K., Karoum, F., Guidotti, A., and Costa, E.: Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells, Nature 283, 489, 1980
牛	牛	Medulla	Collagenase: 0.05%	Locke's solution, CF	Kilpatrick, D., Ledbetter, F., Carson, K., Kirshner, A., Slepetis, R., and Kirshner, N.: Stability of B绵羊 Adrenal Medulla Cells in Culture, J Neurochem 35 (3), 679, 1980
牛	牛, 成年的	Medulla	Collagenase Type 1: 0.5%	HBSS	Unsicker, K., and Griesser, G.: Establishment, Characterization and Fibre Outgrowth of Isolated B绵羊 Adrenal Medullary Cells in Long-Term Cultures,Neuroscience 5, 1445, 1980
牛	牛	Chromaffin	Collagenase: 0.05%	DMEM	Aunis, D., Guerold, B., Bader, M-F., and Cieselski-Treska, J.:Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells, Neuroscience 5, 2261, 1980
牛	牛	Medullary	Collagenase Type 2: 0.2%	HEPES	Hersey, R., and DiStefano, V.: Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured B绵羊 Adrenal Medullary Cells, J Pharmacol Exp Ther 209 (1), 147, 1979
牛	牛	Foreskin	Collagenase: 0.5%	Dulbecco's MEM w/10% calf serum	Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
牛	牛	Medulla	Collagenase Type 1: 0.05%	Kreb's, CF	Fenwick, E., Fajdiga, P., Howe, N., and Livett, B.: Functional and Morphological Characterization of Isolated B绵羊 Adrenal Medullary Cell, J Cell Biol 76, 12, 1978
牛	牛	Chromaffin	Hyaluronidase: 0.2%	HEPES, CF	Brooks, J.C.: The Isolated B绵羊 Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion, Endocrinology 101, 1369, 1977
牛	牛, 雄性	Chromaffin	Collagenase Type 1: 0.2%	Kreb's Ringer bicarbonate buffer, CF	Schneider, A., Herz, R., and Rosenheck, K.: Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla, Proc Natl Acad Sci U S A 74, 5036, 1977
豚鼠	豚鼠, 500-700克	Chromaffin	Collagenase:	BSS	Role, L.W., Leeman, S.E., and Perlman, R.L.: Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of 豚鼠 Adrenal Medulla, Neurochem Int 6, 1813, 1981
豚鼠	豚鼠, 500-700克	Medulla	Collagenase:	(see reference)
豚鼠	豚鼠	Chromaffin	Collagenase: 0.5%	HBSS	Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980
豚鼠	(还有大鼠,汉诺威-威斯塔, 年幼的; 初生; 牛)	Chromaffin	Collagenase: 0.5%	HBSS
豚鼠	豚鼠	Adrenal	Collagenase: 0.05%-0.20%	Kreb's-Ringer bicarb glucose buffer, CF	Hochman, J., and Perlman, R.L.: Catecholamine Secretion by Isolated Adrenal Cells, Biochim Biophys Acta 421, 168, 1976
豚鼠	豚鼠	Chromaffin	Collagenase: 0.05%-0.20%	Kreb's-Ringer bicarb glucose buffer, CF
仓鼠	仓鼠 (金仓鼠) 100-150 克	Adrenal	Hyaluronidase: 0.20%	Kreb's Ringer bicarbonate buffer	Liang, B.T., and Perlman, R.L.: Catecholamine Secretion by 仓鼠 Adrenal Cells, J Neurochem 32, 927, 1979
仓鼠	仓鼠 (金仓鼠) 100-150 克	Chromaffin	Hyaluronidase: 0.20%	Kreb's Ringer bicarbonate buffer
人类	人类	Adrenal medulla progenitor	Collagenase: 0.2%	DMEM/F-12	Santana, M., Chung, K., Vukicevic, V., Rosmaninho-Salgado, J., Kanczkowski, W., Cortez, V., Hackmann, K., Bastos, C., Mota, A., Schrock, E., Bornstein, S., Cavadas, C. and Ehrhart-Bornstein, M.: Isolation, Characterization, and Differentiation of Progenitor Cells from 人类 Adult Adrenal Medulla., Stem Cells Transl Med 1, 783, 2012
人类	人类	Chromaffin cells	Collagenase: 0.2%	Locke's solution	Jeon, Y., Baek, W., Chung, S., Shin, N., Kim, H., and Lee, S.: Cultured 人类 Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model., Korean J Anesthesiol 60, 357, 2011
人类	人类	Adrenocortical	Collagenase Type 1: 0.2%	Krebs Ringer	Caroccia, B., Fassina, A., Seccia, T., Recarti, C., Petrelli, L., Belloni, A., Pelizzo, M. and Rossi, G.: Isolation of 人类 Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method., Endocrinology 151, 1375, 2010
人类	人类	Adrenocortical	Deoxyribonuclease I: 0.01%	Krebs Ringer
人类	人类, 成年的	Chromaffin	Trypsin: 0.25%	Eagle's MEM	Tischler, A., DeLellis, R., Bailes, B., Nunnemacher, G.,Carabba, V., and Wolfe, H.: Nerve Growth Factor-Induced Neurite Outgrowth from Normal 人类 Chromaffin Cells, Lab Invest 43, 399, 1980
人类	人类, 成年的和小孩	Foreskin	Collagenase: 0.5%	Dulbecco's MEM w/10% calf serum	Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
小鼠	小鼠	Chromaffin	Collagenase Type 1: 0.1-0.2%	DMEM/F12	Dominguez, N., Rodri璯uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012
			Deoxyribonuclease I: 0.003-0.015%
			Hyaluronidase: 0.015%
小鼠	小鼠, 8-10 周	Chromaffin	Papain: 40 u/ml	DMEM	Kolski-Andreaco, A., Cai, H., Currle, D., Chandy, K. and Chow, R.: 小鼠 Adrenal Chromaffin Cell Isolation., J Vis Exp 2, 129, 2007
小鼠	小鼠, 胚胎	Chromafin cells	Papain: 20-25 u/ml	DMEM	Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang: The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells, J Neurosci 25, 10546-55, 2005
绵羊	绵羊, 成年的和胚胎	Adrenocortical	Collagenase Type 1: 0.4%	DMEM/Ham's F12	Valego, N. and Rose, J.: A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in 绵羊 Adrenocortical Cells., Reprod Sci 17, 477, 2010
绵羊	绵羊, 胚胎	Adrenocortical	Collagenase Type 1: 0.4%	DMEM/Ham's F12	Valego, N., Su, Y., Carey, L., Young, S., Tatter, S., Wang, J. and Rose, J.:Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression.,Am J Physiol Regul Integr Comp Physiol 289, R410, 2005
绵羊	绵羊, 成年	Chromaffin cells	Collagenase Type 2: 0.2%	Locke's solution	Keating, D., Rychkov, G., Adams, M., Holgert, H., McMillen, I.C. and Roberts, M.: Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels., J Physiol555, 489, 2004
绵羊	绵羊, 成年	Chromaffin cells	Deoxyribonuclease I: 100 u/ml	Locke's solution
绵羊	绵羊, 3 年	Anterior pituitary	Trypsin: 2.5%	DMEM	Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J: The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep, J Endocrinol 162, 215-25, 1999
绵羊	绵羊, 3 年	Anterior pituitary	Deoxyribonuclease I: 0.004%	DMEM
大鼠	大鼠	Chromaffin	Collagenase Type 1: 0.1-0.2%	DMEM/F12	Dominguez, N., Rodri璯uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012
			Deoxyribonuclease I: 0.003-0.015%
			Hyaluronidase: 0.015%
大鼠	大鼠	Chromaffin	Collagenase Type 1: 0.26%	HBSS	Gilabert, J, Montalvo, G, and Artalejo A.: Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays,Nat Protoc , 294, 2006
			Deoxyribonuclease I: 0.015%
			Hyaluronidase: 0.015%
大鼠	大鼠, SD	Chromaffin cells	Collagenase Type 1: 0.26%	HBSS	Gilabert, J: Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture,Cell Biology of the Chromaffin Cell,Borges, R. and Gandia, L., Instituto Teofilo Hernando, , 2004
			Deoxyribonuclease I: 0.015%
			Hyaluronidase: 0.015%
大鼠	大鼠, SD, 雄性	Zona fasciculata/reticularis	Collagenase: 0.4%	Krebs-HEPES	Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel: An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells, Am J Physiol Regul Integr Comp Physiol 284, R1631-5, 2003
大鼠	大鼠, 威斯塔, 初生	Chromaffin cells	Collagenase Type 1: 0.025%	DMEM	Zhang L, Casl A, Avila E, Drucker-Col铆n R, Escobar A:Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons, J Neuropathol Exp Neurol 59, 170-4, 2000
大鼠	大鼠, 威斯塔, 初生	Chromaffin cells	Deoxyribonuclease I: 0.0015%	DMEM
大鼠	大鼠, SD, 雄性	ZG	Collagenase Type 1: 0.2%	Kreb's	Sayed, S., Whitehouse, B., and Jones, P.: Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis, J Endocrinol 154, 449, 1997
大鼠	大鼠, SD, 雄性	ZFR	Collagenase Type 1: 0.2%	Kreb's
大鼠	大鼠, 费希尔, 雄性, 10-16周	Adrenocortical	Deoxyribonuclease I: 0.005%	BSS	Roskelley, C.D. and Auersperg, N.: Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture,In Vitro Cell Dev Biol 26, 493, 1990
大鼠	大鼠, 雄性, 120-160 g,	Leydig	Collagenase Type 2: 0.03% (adrenal)	Krebs Ringer bicarbonate buffer	Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26, 24, 1990
大鼠	大鼠, SD, 雄性, 400-450 g	Adrenal	Collagenase Type 2: 0.03% (adrenal)	Krebs Ringer bicarbonate buffer
大鼠	大鼠, SD, 2-4 day old	Heart	Trypsin: 0.06%	25mM HEPES buffered Locke's solution, CMF	Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B绵羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
	(also 牛)	Adrenal chromaffin
		Paraneurons
大鼠	大鼠, Long-Evans, 雌性, 150-200 g	Glomerulosa	Collagenase: 0.2%	MEM-d-Val	Payet, N., Deziel, Y., and Lehoux, J.-G.: Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture, J Steroid Biochem 20, 449, 1984
大鼠	大鼠, 费希尔, 雄性, 1-10月	Adrenocortical	Deoxyribonuclease I: 0.005%	BSS	Leonard, R.K., Auersperg, N., and Parkes, C.O.: Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells, In Vitro 19, 46, 1983
大鼠	大鼠, SD, 雄性, 400-450 克	Decapular	Deoxyribonuclease I: 0.01%	Medium 199	Li, C.H., Ng, T.B., and Cheng, C.H.K.: Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells, Int J Pept Protein Res 19, 361, 1982
		Capsular
		Glomerulosa
大鼠	大鼠	Chromaffin	Trypsin: 0.10%	Ham's F-12 w/HEPES	Englert, D.F.: An Optical Study of Isolated Rat Adrenal Chromaffin Cells, Exp Cell Res 125, 369, 1980
大鼠	大鼠, 汉诺威-威斯塔, 第二出生后的一周 (还有豚鼠, 牛)	Chromaffin	Collagenase: 0.5%	HBSS	Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells, Adv Biochem Psychopharmacol 25, 51, 1980
大鼠	大鼠 , SD, 雌性, 200 克	Glomerulosa	Deoxyribonuclease I: 0.05%	Kreb's	Braley, L., Williams, G., and Bradwin, G.: The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells, Endocrinology 106 (1), 50, 1980
大鼠	大鼠	Foreskin	Collagenase: 0.5%	Dulbecco's MEM w/10% calf serum	Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
大鼠	大鼠, 汉诺威-威斯塔, 7-12 天	Medullary	Trypsin: 0.125%	HBSS	Unsicker, K., Krisch, B., Otten, U., and Thoenen, H.: Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids, Proc Natl Acad Sci U S A 75 (7), 3498, 1978
大鼠	大鼠, SD, 雄性	Cortical	Trypsin: 0.25%	Kreb's Ringer bicarbonate buffer	Barofsky, A., Feinstein, M., and Halkerston, I.: Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands,Exp Cell Res 79, 263, 1973
大鼠	大鼠, Holtzman, 雄性, 180-250 g	Adrenal	Collagenase Type 1: 0.5%	Kreb's Ringer bicarbonate buffer	Kloppenborg, P., Island, D., Liddle, G., Michelakis, A., and Nicholson, W.: A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism, Endocrinology 82, 1053, 1968

我们公司zui大优势是强大的采购，

1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，

2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，

3：提供加急服务，一般1-2周到货，超过时限加急费全免

4：价格公道，绝大部分价格有优势，当然不能保证100%产品都是,因为意味着没有服务.

5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司

6：我们还是Santa,Advanced Biotechnologies Inc, Worthington Biochemical ,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. Dako,Inc, GePromegaridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,Worthington Biochemical , Polysciences,prospecbi, QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司授权代理。
7：我们还是invitrogen,qiagen,Worthington Biochemical am,sigma;Promega,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发，欢迎合作。

上一条：骨组织消化手册
下一条：脂肪组织消化手册